Publications

  1. Akkerman, V., Scheidt, H.A., Reinholdt, P., Bashawat, M., Szomek, M., Lehmann, M., Wessig, P., Covey, D. F., Kongsted, J., Müller, P. and Wüstner, D. 2023. Natamycin interferes with ergosterol-dependent lipid phases in model membranes. Biochim. Biophys. Acta. Advances. 4, 100102.
  2.  Wagh, K., Stavreva, D.A., Jensen, R.A.M., Paakinaho, V., Fettweis, G., Schiltz, R.L., Wüstner, D., Mandrup, S., Presman, D.M., Upadhyaya, A. and Hager, G.L. 2023. Single molecule tracking reveals two low-mobility states for chromatin and transcriptional regulators in the nucleus. Science. Adv. 9(24)eade1122
  3. Mulberg, M.W., Hornum, M., Reinholdt, P. Jensen, B.B., Szomek, M., Brewer, J. R., Wüstner, D., Kongsted, J. and Nielsen, P. 2023. Facile Suzuki coupling strategy toward new Nile Red derivatives with improved two-photon brightness. Eur. J. Org. Chem. e202300238.
  4. Prioli, S., Wüstner, D. and Kongsted, J., 2022. Computational Analysis of Altered One and Two-Photon CD of Sterols Inside a Protein Binding Pocket. Theoretical Chemistry Accounts 141:5.
  5. Juhl, A.D. and Wüstner, D. 2022. Pathways and mechanisms of cellular cholesterol efflux – insight from imaging. Review. Frontiers Cell Develop. Biol. 362, 10, 834408.
  6. Juhl, A.D., Anvarian, Z., Kuhns, S., Berges J., Andersen, J.S., Wüstner, D. and Pedersen, L.B. 2022. Transient accumulation and bidrectional movement of KIF13B in primary cilia. J. Cell Science. 136, jcs259257.
  7. Wüstner, D., 2022. Dynamic mode decomposition of fluorescence loss in photobleaching microscopy data for model-free analysis of protein transport and aggregation in living cells. Sensors. 22(13), 4731
  8. Wüstner, D. 2022. Image segmentation and separation of spectrally similar dyes in fluorescence microscopy by dynamic mode decomposition of photobleaching kinetics. BMC Bioinformatics. 23, 334.
  9. Szomek, M., Reinholdt, P., Walther, H-L., Scheidt, H., Müller, P., Obermaier, S., Poolman, B., Kongsted, J. and Wüstner, D. 2022. Natamycin sequesters ergosterol and interferes with substrate transport of the lysine transporter Lyp1 from yeast. Biochim. Biophys. Acta. 1864(11), 184012.
  10. Reinholdt P, Joensen LE, Petersen D, Szomek M, Mularski A, Simonsen AC, Kongsted J, Wüstner D. Photophysical and Structural Characterization of Intrinsically Fluorescent Sterol Aggregates. J Phys Chem B. 2021 Jun 1. PMID: 34061522.
  11. Juhl AD, Heegaard CW, Werner S, Schneider G, Krishnan K, Covey DF, Wüstner D.
    Quantitative imaging of membrane contact sites for sterol transfer between endo-lysosomes and mitochondria in living cells. Sci Rep. 2021 Apr 26. PMID: 33903617.
  12. Juhl AD, Lund FW, Jensen MLV, Szomek M, Heegaard CW, Guttmann P, Werner S,
    McNally J, Schneider G, Kapishnikov S, Wüstner D. “Niemann Pick C2 protein enables cholesterol transfer from endo-lysosomes to the plasma membrane for efflux by shedding of extracellular vesicles.” Chem Phys Lipids. 2021 Mar. PMID: 33422548. 
  13. Hornum M, Mulberg MW, Szomek M, Reinholdt P, Brewer JR, Wüstner D, Kongsted J, Nielsen P. Substituted 9-Diethylaminobenzo[a]phenoxazin-5-ones (Nile Red Analogues): Synthesis and Photophysical Properties. J Org Chem. 2021 Jan. PMID: 33370098.
  14. Work EM, Ferraudi G, Kiefer L, Liu G, Grigalunas M, Bhardwaj A, Kaur R, Dempsey JM, Wüstner D, Helquist P, Wiest O. Design, Synthesis, and Evaluation of a Luminescent Cholesterol Mimic. J Org Chem. 2021 Jan. PMID: 33369429.
  15. Direct observation of nystatin binding to the plasma membrane of living cells. Szomek M, Reinholdt P, Petersen D, Caci A, Kongsted J, Wüstner D. Biochim Biophys Acta Biomembr. 2020 Dec 3;1863(2):183528. PMID: 33279513.
  16. Moesgaard L, Petersen D, Szomek M, Reinholdt P, Winkler MBL, Frain KM, Müller P, Pedersen BP, Kongsted J, Wüstner D. Mechanistic Insight into Lipid Binding to Yeast Niemann Pick Type C2 Protein. Biochemistry. 2020 Nov 3. PMID: 33141558
  17. Szomek M, Moesgaard L, Reinholdt P, Haarhøj Hald SB, Petersen D, Krishnan K, Covey DF, Kongsted J, Wüstner D. Membrane organization and intracellular transport of a fluorescent analogue of 27-hydroxycholesterol. PLoS Comput Biol. 2020 Oct 6;16(10):e1007554. PMID: 33021976
  18. Dubey V, Bozorg B, Wüstner D, Khandelia H. Cholesterol binding to the sterol-sensing region of Niemann Pick C1 protein confines dynamics of its N-terminal domain. PLoS Comput Biol. 2020 Oct 6;16(10):e1007554. PMID: 33021976.
  19. Hornum M , Reinholdt P , Zaręba JK , Jensen BB , Wüstner D , Samoć M , Nielsen P , Kongsted J. One- and two-photon solvatochromism of the fluorescent dye Nile Red and its CF3, F and Br-substituted analogues. Photochem Photobiol Sci. 2020 Oct 1;19(10):1382-1391. PMID: 32869822. 
  20. Moesgaard L, Reinholdt P, Wüstner D, Kongsted J. Modeling the Sterol-Binding Domain of Aster-A Provides Insight into Its Multiligand Specificity. J Chem Inf Model. 2020;60(4):2268-2281. PMID: 32233488.   
  21. Poongavanam V, Kongsted J, Wüstner D. Computational Modeling Explains the Multi Sterol Ligand Specificity of the N-Terminal Domain of Niemann-Pick C1-Like 1 Protein. ACS Omega. 2019;4(25):20894-20904. PMID: 31867479. 
  22. Winkler, M. B. L., et al. (2019). Structural Insight into Eukaryotic Sterol Transport through Niemann-Pick Type C Proteins. Cell 179(2): 485-497.e418.
  23. Petersen, D., et al. (2019). Binding and intracellular transport of 25-hydroxycholesterol by Niemann-Pick C2 protein. Biochim Biophys Acta Biomembr: 183063.
  24. Wüstner D. Steady state analysis of influx and transbilayer distribution of ergosterol in the yeast plasma membrane. Theor Biol Med Model. 2019 Aug 15;16(1):13. PubMed PMID: 31412941.
  25. Reinholdt P, Wind S, Wüstner D, Kongsted J. Computational Characterization of a Cholesterol-Based Molecular Rotor in Lipid Membranes. J Phys Chem B. 2019 Aug 29;123(34):7313-7326.PubMed PMID: 31381343. 
  26. Bonvicini A, Reinholdt P, Tognetti V, Joubert L, Wüstner D, Kongsted J. Rational design of novel fluorescent analogues of cholesterol: a “step-by-step” computational study. Phys Chem Chem Phys. 2019 Jul 17;21(28):15487-15503. PubMed PMID: 31259332.
  27. Stendevad J, Hornum M, Wüstner D, Kongsted J. Photophysical investigation of two emissive nucleosides exhibiting gigantic stokes shifts. Photochem Photobiol, Sci. 2019 Jul 10;18(7):1858-1865. doi: 10.1039/c9pp00172g. PubMed PMID: 31231745. 
  28. Hansen CV, Schroll HJ, Wüstner D. A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates. BMC Biophys. 2018 Nov 29;11:7. doi: 10.1186/s13628-018-0046-0. eCollection 2018. PubMed PMID: 30519460; PubMed Central PMCID: PMC6264036.
  29. Krog, J., Jacobsen, L.H., Lund, F.W., Wüstner, D. and Lomholt, M.A. 2018.
    Bayesian model selection with fractional Brownian motion. 2018. J. Stat.
    Physics, 9,093501. 
  30. Berzina Z, Solanko LM, Mehadi AS, Jensen MLV, Lund FW, Modzel M, Szomek M,Solanko KA, Dupont A, Nielsen GK, Heegaard CW, Ejsing CS, Wüstner D. Niemann-Pick C2 protein regulates sterol transport between plasma membrane and late endosomes in human fibroblasts. Chem Phys Lipids. 2018 Jul;213:48-61. PubMed PMID: 29580834.
  31. Hansen CV, Schroll HJ, Wüstner D. A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching. Sci Rep. 2018 Jan 23;8(1):1387. doi: 10.1038/s41598-018-19159-7. PubMed PMID: 29362364; PubMed Central PMCID: PMC5780497. 
  32. Nåbo LJ, Modzel M, Krishnan K, Covey DF, Fujiwara H, Ory DS, Szomek M, Khandelia H, Wüstner D, Kongsted J. Structural design of intrinsically fluorescent oxysterols. Chem Phys Lipids. 2018 May;212:26-34. doi:10.1016/j.chemphyslip.2017.12.005. Epub 2017 Dec 26. PubMed PMID: 29287905.
  33. Solanko LM, Sullivan DP, Sere YY, Szomek M, Lunding A, Solanko KA, Pizovic A, Stanchev LD, Pomorski TG, Menon AK, Wüstner D. Ergosterol is mainly located in the cytoplasmic leaflet of the yeast plasma membrane. Traffic. 2018 Mar;19(3):198-214. doi: 10.1111/tra.12545. PubMed PMID: 29282820.
  34.  Modzel, M., Solanko, K. A., Szomek, M., Hansen, S. K., Dupont, A., Nabo, L.
    J., Kongsted, J., and Wüstner, D. 2018 Live-cell imaging of new polyene
    sterols for improved analysis of intracellular cholesterol transport. J. Microsc.,
    271(1),36-48. 
  35. Lund FW, Wüstner D. Quantitative Co-Localization and Pattern Analysis of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets.
    Methods Mol Biol. 2017;1594:93-128. PubMed PMID: 28456978.
  36. Modzel M, Lund FW, Wüstner D. Synthesis and Live-Cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport. Methods Mol Biol. 2017. PubMed PMID: 28205171.
  37. Nåbo LJ, Olsen JM, Holmgaard List N, Solanko LM, Wüstner D, Kongsted J. Embedding beyond electrostatics-The role of wave function confinement. J Chem Phys. 2016 Sep 14;145(10):104102. doi: 10.1063/1.4962367. PubMed PMID: 27634246. 
  38. Poongavanam V, Kongsted J, Wüstner D. Computational Analysis of Sterol Ligand Specificity of the Niemann Pick C2 Protein. Biochemistry. 2016 Sep 13;55(36):5165-79. PubMed PMID: 27533706.
  39. Wüstner D, Modzel M, Lund FW, Lomholt MA. Imaging approaches for analysis of cholesterol distribution and dynamics in the plasma membrane. Chem Phys Lipids. 2016 Sep;199:106-135. PubMed PMID: 27016337.
  40. Sezgin E, Can FB, Schneider F, Clausen MP, Galiani S, Stanly TA, Waithe D, Colaco A, Honigmann A, Wüstner D, Platt F, Eggeling C. A comparative study on fluorescent cholesterol analogs as versatile cellular reporters. J Lipid Res. 2016 Feb;57(2):299-309. PubMed PMID:26701325.
  41. Wüstner D, Lund FW, Röhrl C, Stangl H. Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28.  PubMed PMID: 26291493.
  42. Wüstner D, Solanko K. How cholesterol interacts with proteins and lipids during its intracellular transport. Biochim Biophys Acta. 2015 Sep;1848(9):1908-26. PubMed PMID: 26004840.
  43. Nåbo LJ, List NH, Witzke S, Wüstner D, Khandelia H, Kongsted J. Design of new fluorescent cholesterol and ergosterol analogs: Insights from theory. Biochim Biophys Acta. 2015 Oct. PubMed PMID: 25963993.
  44. Solanko KA, Modzel M, Solanko LM, Wüstner D. Fluorescent Sterols and Cholesteryl Esters as Probes for Intracellular Cholesterol Transport. Lipid Insights. 2016 Jun. Review. PubMed PMID: 27330304. 
  45. Lund FW, Jensen ML, Christensen T, Nielsen GK, Heegaard CW, Wüstner D. SpatTrack: an imaging toolbox for analysis of vesicle motility and distribution in living cells. Traffic. 2014 Dec;15(12):1406-29. PubMed PMID: 25243614.
  46. Wüstner D, Christensen T, Solanko LM, Sage D. Photobleaching kinetics and time-integrated emission of fluorescent probes in cellular membranes. Molecules. 2014 Jul 29;19(8)PubMed PMID: 25076144.
  47. Pourmousa M, Róg T, Mikkeli R, Vattulainen L, Solanko LM, Wüstner D, List NH, Kongsted J, Karttunen M. Dehydroergosterol as an analogue for cholesterol: why it mimics cholesterol so well-or does it? J Phys Chem B. 2014 Jul. PubMed PMID: 24893063.
  48. Wüstner D, Sklenar H. Atomistic Monte Carlo simulation of lipid membranes. Int J Mol Sci. 2014 Jan 24;15(2):1767-803. Review.PubMed PMID: 24469314.
  49. Solanko LM, Honigmann A, Midtiby HS, Lund FW, Brewer JR, Dekaris V, Bittman R, Eggeling C, Wüstner D. Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure. Biophys J. 2013 Nov 5;105(9):2082-92. PubMed PMID: 24209853. 
  50. Wüstner D, Solanko LM, Lund FW, Sage D, Schroll HJ, Lomholt MA. Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation. BMC Bioinformatics. 2012 Nov 13;13:296. PubMed PMID: 23148417.
  51. Lund FW, Lomholt MA, Solanko LM, Bittman R, Wüstner D. Two-photon time-lapse microscopy of BODIPY-cholesterol reveals anomalous sterol diffusion in chinese hamster ovary cells. BMC Biophys. 2012 Oct 18;5:20. PubMed PMID: 23078907.
  52. Maxfield FR, Wüstner D. Analysis of cholesterol trafficking with fluorescent probes.
    Methods Cell Biol. 2012. PubMed PMID: 22325611.
  53. Wüstner D. Following intracellular cholesterol transport by linear and non-linear optical microscopy of intrinsically fluorescent sterols. Curr Pharm Biotechnol. 2012 Feb;13(2):303-18. Review. PubMed PMID: 21470123.
  54. Wüstner D, Solanko L, Sokol E, Garvik O, Li Z, Bittman R, Korte T, Herrmann A. Quantitative assessment of sterol traffic in living cells by dual labeling with dehydroergosterol and BODIPY-cholesterol. Chem Phys Lipids. 2011 Mar;164(3):221-35. PubMed PMID: 21291873.
  55. Wüstner D, Brewer JR, Bagatolli L, Sage D. Potential of ultraviolet wide-field imaging and multiphoton microscopy for analysis of dehydroergosterol in cellular membranes. Microsc Res Tech. 2011 Jan;74(1):92-108. PubMed PMID: 21181715.
  56. Kohut P, Wüstner D, Hronska L, Kuchler K, Hapala I, Valachovic M. The role of ABC proteins Aus1p and Pdr11p in the uptake of external sterols in yeast:dehydroergosterol fluorescence study. Biochem Biophys Res Commun. 2011 Jan. PubMed PMID: 21110944.
  57. Wüstner D, Sage D. Multicolor bleach-rate imaging enlightens in vivo sterol transport.
    Commun Integr Biol. 2010 Jul;3(4):370-3. PubMed PMID: 20798830. 
  58. Wüstner D, Landt Larsen A, Faergeman NJ, Brewer JR, Sage D. Selective visualization of fluorescent sterols in Caenorhabditis elegans by bleach-rate-based image segmentation.
  59. Traffic. 2010 Apr;11(4). PubMed PMID: 20070610.
    Garvik O, Benediktson P, Simonsen AC, Ipsen JH, Wüstner D. The fluorescent cholesterol analog dehydroergosterol induces liquid-ordered domains in model membranes. Chem Phys Lipids. 2009 Jun;159(2):114-8. PubMed PMID: 19477318.
  60. Benninger RK, Vanherberghen B, Young S, Taner SB, Culley FJ, Schnyder T, Neil MA, Wüstner D, French PM, Davis DM, Onfelt B. Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. Biophys J. 2009 Jan;96(2):L13-5. PubMed PMID: 19167281,
  61. Wüstner D, Faergeman NJ. Spatiotemporal analysis of endocytosis and membrane distribution of fluorescent sterols in living cells. Histochem Cell Biol. 2008 Nov;130(5):891-908.PubMed PMID: 18787836.
  62. Wüstner D, Faergeman NJ. Chromatic aberration correction and deconvolution for UV sensitive imaging of fluorescent sterols in cytoplasmic lipid droplets. Cytometry A. 2008 Aug;73(8):727-44. PubMed PMID: 18561197.
  63. Wüstner D. Free-cholesterol loading does not trigger phase separation of the fluorescent sterol dehydroergosterol in the plasma membrane of macrophages. Chem Phys Lipids. 2008 Aug;154(2):129-36. PubMed PMID: 18534190.
  64. Petersen NH, Faergeman NJ, Yu L, Wüstner D. Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells. J Lipid Res. 2008 Sep;49(9):2023-37. PubMed PMID: 18523240.
  65. Wüstner D. Fluorescent sterols as tools in membrane biophysics and cell biology. Chem Phys Lipids. 2007 Mar;146(1):1-25. Review. PubMed PMID: 17241621.
  66. Wüstner D. Plasma membrane sterol distribution resembles the surface topography of living cells. Mol Biol Cell. 2007 Jan;18(1):211-28.PubMed PMID: 17065557.
  67. Wüstner D. Quantification of polarized trafficking of transferrin and comparison with bulk membrane transport in hepatic cells. Biochem J. 2006 Dec 1;400(2):267-80. PubMed PMID: 16879100.
  68. Wüstner D. Steady state analysis and experimental validation of a model for hepatic high-density lipoprotein transport. Traffic. 2006 Jun;7(6):699-715. PubMed PMID: 16637891.
  69. Sklenar H, Wüstner D, Rohs R. Using internal and collective variables in Monte Carlo simulations of nucleic acid structures: chain breakage/closurealgorithm and associated Jacobians. J Comput Chem. 2006 Feb;27(3):309-15. PubMed PMID: 16355439.
  70. Wüstner D, Mondal M, Tabas I, Maxfield FR. Direct observation of rapid internalization and intracellular transport of sterol by macrophage foam cells. Traffic. 2005 May;6(5):396-412. PubMed PMID: 15813750.
  71. Wüstner D. Mathematical analysis of hepatic high density lipoprotein transport based on quantitative imaging data. J Biol Chem. 2005 Feb 25;280(8):6766-79. Epub 2004 Dec 17. PubMed PMID: 15613466.
  72. Wüstner D, Mondal M, Huang A, Maxfield FR. Different transport routes for high density lipoprotein and its associated free sterol in polarized hepatic cells. J Lipid Res. 2004 Mar;45(3):427-37. Epub 2003 Dec 16. PubMed PMID:14679167.
  73. Tannert A, Wüstner D, Bechstein J, Müller P, Devaux PF, Herrmann A. Aminophospholipids have no access to the luminal side of the biliary canaliculus:implications for thr specific lipid composition of the bile fluid. J Biol Chem.2003 Oct 17;278(42):40631-9. Epub 2003 Aug 6. PubMed PMID: 12904307.
  74. Maxfield FR, Wüstner D. Intracellular cholesterol transport. J Clin  Invest.2002 Oct;110(7):891-8. Review. PubMed PMID: 12370264; PubMed Central PMCID:PMC151159.
  75. John K, Kubelt J, Müller P, Wüstner D, Herrmann A. Rapid transbilayer movement of the fluorescent sterol dehydroergosterol in lipid membranes. Biophys J. 2002 Sep;83(3):1525-34. PubMed PMID: 12202377. 
  76. Wüstner D, Herrmann A, Hao M, Maxfield FR. Rapid nonvesicular transport of sterol between the plasma membrane domains of polarized hepatic cells. J Biol Chem. 2002 Aug 16;277(33):30325-36. PubMed PMID: 12050151.
  77. Hao M, Lin SX, Karylowski OJ, Wüstner D, McGraw TE, Maxfield FR. Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle. J Biol Chem. 2002 Jan 4;277(1):609-17. PubMed PMID: 11682487.